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Antifungal activity of ethanol extract wild mushroom Agaricus bitorqui ( Quel ) Sacc.

Nemanja Kucljak
Centar za mlade talente Beograd II, Beograd, kucljak.nemanja@gmail.com

Table

Introduction Fungi mg/ml A.bitorquis Ketoc Bifon
Fungi are part of large and complex communities of Et
organisms that includes: lichens, yeasts, molds, edible, A. fumigatus MIC 3.120 0.200 0.150
inedible and poisonous mushrooms. The world in which MFC 6.250 0.500 0.200
we live would be incomparably different if there were no A. versicolors MIC 0.390 0.200 0.100
MFC
3.120
0.200
0.500
fungi. The complex life cycle of the fungus also makes it A. ochraceus MIC 0.390 0.150 0.150
difficult to estimate their numbers. Mushrooms that are MFC 3.120 0.200 0.200
used in the diet are characterized by excellent taste and A. niger MIC 1.560 0.200 0.150
aroma; they are healthy foods and are extremely rich in MFC 6.250 0.500 0.200
amino acids, vitamins and minerals, and have a high water T. viride MIC 0.780 0.200 0.100
3.120
0.300
MFC
0.200
content and beneficial effect on human metabolism. P. funiculosum MIC 1.560 2.500 0.200
Representatives of the fungi are used in the treatment of MFC 3.120 3.500 0.250
various diseases, medicinal fungi are very important P. ochrochloron MIC 1.560 0.200 0.200
because they are rich in biologically active compounds MFC 3.120 0.500 0.250
(secondary metabolites) some of which are identified as P. aurantiogriseum MIC 1.560 1.000 0.150
MFC
0.200
1.000
6.250
active molecules.

Materials and Methods From the table (1) we can see that the extract of A. bitorquis showed
good antifungal activity somewhere even better than synthetic antifungal
drugs.
The paper used fruiting bodies of mushrooms city
A.bitorquis collected in June 2014 in the locality of
Mirijeva (near Belgrade).
Fungi were identified based on the keys and literature (1),
lyophilized (LH Leybold, Lyovac GT2, Frenkendorf,
Switzerland) and crushed to a fine powder. Total ethanol
extract powder (2) was used for in vitro testing
microdilution method, 96 - fungal defense system (3).
Results and Discussion

Based on these results it can be concluded that the
different types Micromycete differently sensitive to
ethanol extract of the city mushrooms. The greatest
resistance showed the fungus A. fumigatus, A. niger and P.
aurantiogriseum, while the most sensitive fungus T. viride.
Comparing the antimicrobial activity of extracts and
synthetic antifungal drugs may be noted that the extract Conclusion
had the same or similar inhibitory values in some
Micromycete. In everyday life, people are in constant
contact with fungal.They are so widespread in our The extract showed good antifungal activity against all the
test fungi at the micro-dose-dependent manner. It was
environment to thousands of spores inhaled or enter the
body through ingestion every day. Applying of the city of concluded that the extract of wild mushrooms Agaricus
mushrooms Agaricus bitorquis in the diet could certainly bitorquis can be a very good source of antifungal
substances
have a beneficial effect on the human body in conditions
when exposed to different types of fungal infections. Literature
Excessive use of commercial antifungal drugs in the (1) Hadžić, I. (2012): Priručnik za branje jestivih i
treatment of humans and animals has led to microorganism lekovitih gljiva, EDICIJA, Beograd
resistence. The search for new substances refers to edible (2) Cheng et al. (2008)
fungi as yet insufficiently explored and promising.
(3) Douk, K.D., Dagher, M.S., Sattout, J.E., 1995.
Antifungal activity of the essential oil of Origanum

syriacum L. Journal of Food Protection 58, 1147–1149