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ODREĐIVANJE NIKOTINA U KOSI PUŠAĈA
NICOTINE DETERMINATION IN HAIR OF SMOKERS

Autor:ALEKSA HADŽI-RISTID, učenik II razreda XIII Beogradske gimnazije

Mentor: MARIJA RAKID LJUBOMIROVID

REZIME

Cilj ovog rada jeste odreĎivanje prisustva nikotina u kosi pušača, budući da je nikotin
dobro poznat indikator za kontrolisanje dugog izlaganja duvanskom dimu iz okoline i
utvrĎivanja stastusa aktivnih pušača. Nikotin nije bilo moguće odrediti kvantitativno, jer nije
korišćen interni standard neophodan za precizno odreĎivanje koncentracije nikotina u kosi,
validaciju uzoraka i kontrolu kvaliteta. UraĎen je test na nikotin iz kose, koji obuhvata
sakupljanje uzoraka, alkalnu digestiju, ekstrakciju uzorka i intrumentalno merenje pomoću gasne
hromatografije-masene spektrometrije (GH/MS). Dokazano je prisustvo nikotina u kosi, ali je
nikotin naĎen u tragovima. UtvrĎeno je da su površine GH signala ili pikova nikotina u korelaciji
sa brojem cigareta koje davaoci uzoraka kose u proseku dnevno puše.

Ključne reči: nikotin, kosa, GH/MS, duvanski dim, štetno dejstvo
SUMMARY

The aim for this study was determination of nicotine in smokers’ hair, whereas nicotine is
a well-known indicator for monitoring long-term exposure to environmental tobacco smoke and
for maintaining active smokers’ status. Quantitation of nicotine in hair was impossible, because
we didn’t use internal standard that is needed for precisely determination of hair nicotine
concentration, validation of samples, and quality control. Hair nicotine assay was performed,
including collecting samples, alkaline digestion, extraction of nicotine and experimental method
of gas chromatography-mass spectrometry (GC/MS). Present of nicotine in hair was proved, but
nicotine was found only in traces. It was determined that the areas of chromatogram peaks are in
correlation with the number of cigarettes smoked daily by smokers who gave their samples of
hair.

Key words: nicotine, hair, GC/MS, tobacco smoke, detrimental effect

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