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UTICAJ RAZLIĈITIH KONCENTARACIJA KARBAMID PEROKSIDA NA
STREPTOCOCCUS MUTANS

THE EFFECT OF DIFFERENT CONCETRATATIONS CARMABIDE PEROXIDE ON

STREPTOCOCCUS MUTANS

Autor: TANJA IVANOV, učenica III razreda Zubotehničke škole, Regionalni centar za talente Beograd 2

Mentori: Dr Milena Jovanovid. Stomatološki fakultet, Univerzitet u Beogradu Prof. Dr Dušan Pavlica.
Stomatološki fakultet, Univerzitet u Beogradu

REZIME

U radu je ispitivan uticaj karbamid peroksida na kariogenu vrstu oralnih streptokoka, Str. mutans.

Utvrđivanje antibakterijskog efekta karbamid peroksida obavljeno je disk difuzionom metodom, pri čemu
je korišden soj vrste Str. mutans izolovan iz usne duplje dobrovoljca. Diskovi filter papira natopljenih

različitim koncentracijama karbamid peroksida (10%, 16%, 20%) postavljani su na podlogu TYC-SB agara
na koju je predhodno gusto zasejana vrsta Str. mutans. Nakon dvadesetočasovne inkubacije koja je

obavljana u anaerobnim uslovima, na 37ºC u termostatu, izvršeno je merenje dužine prečnika zone
inhibicije porasta bakterijskih kolonija oko diskova filter papira. Obradom dobijenih rezultata utvrđeno je

postojanje antibakterijske aktivnosti karbamid peroksida prema Str. mutans vrsti. Dužine prečnika zona
inhibicija rasta bakterija oko diskova natopljenih karbamid peroksidom statistički su se značajno

razlikovale u pogledu njegovih različitih koncentracija.

Ključne reči: Karbamid peroksid, Str. mutans, beljenje zuba

SUMMARY
The effect of carbamide peroxide on a cariogenic species of oral streptoccoci, Str. mutans, was

investigated. The antimicrobial activity of carbamide peroxide was examined by using the disk diffusion
method.The strain of Str. mutans that was used was isolated from the oral cavity of a volunteer. Filter

paper disks were soddened by different concentrations of carbamide peroxide (10%, 16%, 20%) and
placed on a plate containing TYC-SB agar, that has been priorly inoculated with Str. mutans, which would
grow and form a lawn of growth. The plates were incubated for 20 hours on 37ºC in anaerobic conditions.

Using a metric ruler, the diameter of the zone of inhibition around each filter paper disk was measured.

After analyzing the results, it can be concluded that carbamide peroxide has an antimicrobial
effect on Str. mutans. The higher concentrations of the agent had a better antimicrobial effect than lower

concentrations, which was proven by statistical analysis of the results.

Key words: Carbamide peroxide, Streptococcus mutans, teeth bleaching

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