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POVECANJE KONCENTRACIJE HAPTOGLOBINA I ALFA2-MAKROGLOBULINA U

SERUMU PACOVA TOKOM AKUTNO FAZNOG ODGOVORA

THE INCREASE OF HAPTOGLOBIN AND ALPHA2-MACROGLOBULIN
CONCENTRATIONS IN RAT SERUM DURING THE ACUTE PHASE RESPONSE

Autori rada: Ana i Nikola, Mentor: dr Mirjana Mihailovid

REZIME

Različite vrste povreda pokredu univerzalan i evolutivno očuvan mehanizam odbrane organizma poznat
kao akutno fazni odgovor (AFO). U ovoj sistemskoj reakciji organizma važnu ulogu ima jetra, u kojoj se
sintetše grupa proteina, poznatih kao akutno fazni proteini (AFP). Dva najznačajnija AFP kod pacova su
alfa 2-makroglobulin (MG) i haptoglobin (Hp). Cilj ovog rada je bio da se metodom imunoelektroforeze
odredi relativna količina MG i Hp u serumu pacova nakon inducije AFO terpentinom, kao
eksperimentalnom modelu AFO. Pradenjem kinetike (6, 12 i 24 sata nakon indukcije AFO), uočava se da
se relativna količina oba izučavana AFP povedava sa vremenom nakon indukovane povrede, da bi
maksimalan nivo bio zabeležen u 24. satu. To je u skladu sa literaturom, jer od 24 do 48 sata nakon AFO
dostiže plato AFP u cirkulaciji, nakon čega opada. U tom periodu, MG, kao protein koji inhibira proteaze
oslobođene iz delija na mestu povrede, štiti tkiva od dalje destrukcije, a Hp sakuplja oslobođeni
hemoglobin iz liziranih eritrocita i sprečava dalji gubitak gvožđa iz organizma.

Ključne reči: haptoglobin, alfa2-makroglobulin, akutno fazni odgovor, pacov

ABSRACT

Different types of trauma induce universal and evolutionary conserved protective mechanism in
organism known as acute phase response (APR). During this systematic reaction liver has important role
producing set of liver-derived proteins, termed acute phase proteins (APP). Two most important
members of APP in rat are alpha2-macroglobulin (MG) and haptoglobin (Hp). The aim of this work was to
determinate the relative MG and Hp concentrations in rat serum after turpentine APR induction by
immnuoelectrophoresis. The results revealed the time-course increase of MG and Hp concentrations in
rat serum after APR induction. The highest level was observed 24 hours after turpentine treatment. This
result is in correlation with literature data showing the highest level of APP in circulation 24-48 hours
after the induction of APR, when the APP concentrations decrease. In this period, MG, as inhibitor of
proteases released from the injured cells, protect tissue from further destruction. The role of Hp is
binding of hemoglobin after haemolysis in order to prevent the loss of iron from organism.

Key words: haptoglobin, alpha2-macroglobulin, acute phase response, rat