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TUMOR SUPRESOR PROTEIN P53 U AKUTNO FAZNOM ODGOVORU I AKUTNOJ

RADIJACIONOJ POVREDI

TUMOR SUPRESSOR P53 IN ACUTE PHASE RESPONSE AND ACUTE RADIATION

DISEASE

Autor rada: Ivana Nedeljkovid, Regionalni centar za talente, Beograd, Mentor: dr Mirjana Mihailovid

REZIME

Akutno fazni odgovor (AFO) predstavlja ranu i nespecifičnu reakciju organizma na različita oštedenja tkiva i infekcije
koja ima za cilj ponovno uspostavljanje narušene homeostaze. Izlaganje celog organizma jonizujudem zračenju u
dozama višim od 1 Gy dovodi do oboljenja koje se ispoljava oštedenjem različitih tkiva i organa poznatom pod
nazivom akutna radijaciona bolest (ARB). Protein p53 je transkripcioni faktor koji učestvuje u regulaciji različitih

gena čiji su produkti direktni i/ili indirektni medijatori delijskih mehanizama, koji se aktiviraju u različitim vrstama
stresa. Cilj ovog rada je bio da se metodom imuno-blot analize odredi prisustvo relativne količine proteina p53 u
jedarnim proteinima jetre pacova tokom AFO i ARB. Rezulati su pokazali da se količine p53 u jetri pacova nakon AFO

slabo indukuje u poređenju sa kontrolom, dok je povedanje ovog proteina tokom ARB značajno uvedano. Razlika u
količini p53 u AFO i ARB je upravo razlika u prirodi oštedenja koja se javljaju u ova dva model sistema povrede
organizma. Oštedenje DNK je jedan od najintenzivnijih stimulatora p53, a ova oštedenja su značajna posledica
izlaganja organizma zračenju, što se reflaktuje i na relativnu količinu proteina p53 u jetri tokom AFO i ARB.

Ključne reči: protein p53, akutno fazni odgovor, akutna radijaciona povreda

ABSTRAKT

The acute phase response (AFR) represent an early and nonspecific response of organisam on different kind of
tissue injures and infections with the aim to restore disturbed homeostasis. Exposure of organism to irradiation
higher than 1 Gy leads to differnt degrees of tissue and organ injures known as acute radiation desease (ARD).
Protein p53 is transcription factor that regulates different genes that are activated in different types of stress. The

aim of this work was to determina te the relative amount of p53 in rat liver nuclear proteins during APR and ARD by
immuno-blot analysis. The results revealed that the induction of p53 during APR is slightly higher compared to the
control level, while the ARD induced significant increase of p53. APR and ARD represents two different model

systems of trauma and different expression of p53 is the consecuense of differences in the degree of injury during
APR and ARD. DNA damage is one of the most important inducer of p53 expression and DNA damage is very high
after irradiation exposure that reflects on the level of p53 during APR and ARD.

Key words: p53 protein, acute phase response, acute radiation desease