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ANTIOKSIDATIVNI KAPACITET BILJKE
VERBASCUM THAPSUS L.

ANTIOXIDANT CAPACITY OF
VERBASCUM THAPSUS L.
Autor: INES PAVLOV, učenica III razreda Gimnazije Mentori: Dr BILJANA KUKAVICA Dipl. biol. TIJANA
CVETID

Rezime

U eksperimentima je korišdena samonikla biljka Verbascum thapsus L. koja je rasla na
zemljištu zagađenom industrijskim otpadom. Da bi preživela na ovakvom zemljištu sa visokom
koncentracijom teških metala, biljka mora da razvije posebne odbrambene mehanizme
(antioksidativni kapacitet). Cilj ovog istraživanja je bio određivanje antioksidativnog kapaciteta u
listu i korenu verbaskuma i poređenje sa antioksidativnim kapacitetom graška koji je rastao u
normalnim uslovima. Na osnovu standardne krive za askorbinsku kiselinu određen je
antioksidativni kapacitet u listu i korenu verbaskuma i graška. Iz rezultata se vidi da znatno vedi
antioksidativni kapacitet imaju koren i list verbaskuma. Apsorpcioni spektri su pokazali da mali
molekuli koji čine antioksidativni kapacitet verbaskuma imaju maksimum apsorpcije u UV oblasti

na osnovu čega se može zaključiti da su to askorbinska kiselina i fenoli. Dakle, verbaskum sadrži
u sebi veliki broj malih molekula koji mu omogudavaju život na zemljištu koje sadrži veliku
količinu teških metala, jer se uspešno bori protiv oksidacije.

Ključne reči: Verbascum thapsus, antioksidanti, antioksidativni kapacitet, askorbat, ABTS test

Summary
In these experiments I have used a wild plant, Verbascum Thapsus (L), which grew on the
soil polluted with industrial waste. In order to survive on the soil with such high concentration of
heavy metals, this plant had to develop special defence mechanisms one of which is antioxidant
capacity. The aim of this research was to define antioxidant capacity in the leaf and root of the
verbaskum and to compare it with the antioxidant capacity of the pea grown in normal
conditions. By using the standard curved line for ascorbic acid, antioxidant capacity was defined
in the leaf and root of the verbaskum as well as in the pea. The results show that the leaf and root
of the verbaskum have significantly larger oxidant capacity. Absorption spectra show that two
small molecules, which comprise antioxidant capacity of the verbaskum, have maximum
absorption in UV part of the spectrum and that leads me to the conclusion that these two
molecules are ascorbic acid and phenol. Thus Verbascum Thapsus survives on the soil polluted
with heavy metals owing to a great number of small molecules which successfully fight against
oxidization.

Key words: Verbascum thapsus, antioxidant, antioxidant capacity, ascorbat, ABTS test