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ANALIZA GENOTOKSIĈNOSTI REĈNE VODE ALLIUM

ANAFAZNO-TELOFAZNIM TESTOM

THE ANALYSIS OF GENOTOXICITY OF RIVER WATER USING ALLIUM

ANAPHASE-TELOPHASE TEST

Autor: GORANA JENDRIŠEK
učenica četvrtog razreda ,,Prve beogradske gimnazije”
Mentor: dr MLADEN VUJOŠEVIĆ Insitut za biološka istraživanja ,,Siniša Stanković”
Beograd
REZIME

Allium anafazno-telofazni test se preporučuje za monitoring i može da posluži za otkrivanje prisustva
genotoksičnih zagaĎivača životne sredine. Veoma je važno utvrditi procenat genotoksičnosti reka, koje
služe kao izvor pitke vode. Prikupljena su tri uzoraka vode 21. januara 2013. iz Save kod ušća u Dunav,

Dunava kod Zemuna i Dunava kod 25. maja, i analizirana Allium anafazno–telofaznim testom, kako bi se
otkrili potencijalni toksični i genotoksični efekti. Nije zabeležena značajna inhibicija rasta korena u

odnosu na negativnu kontrolu u svim uzorcima. Analiza genotoksičnih efekata, prebrojavanjem aberacija
u anafazama i telofazama, pokazala je da se svi uzorci značajno razlikuju u odnosu na pozitivnu kontrolu
(metilmetansulfonat), dok se samo jedan uzorak vode iz Save značajno razlikovao u odnosu na negativnu

kontrolu.

Kljuĉne reĉi: genotoksičnost, Allium test, zagaĎenje, kvalitet vode

SUMMARY

Allium anaphase–telophase test is recommended for monitoring and can be used to detect the presence of
genotoxic environmental pollutants. It is very important to determine the percentage of genotoxicity of
th
river water, which serves as a source of drinking water. Three water samples were collected on 21 of
January 2013 from the confluence of the Sava and Danube rivers, the Danube near Zemun and Danube at
Sport center 25. May, and analyzed using the Allium anaphase–telophase test, in order to detect potential
toxic and genotoxic effects. Significant inhibition of root growth in relation to the negative control was not

recorded. Analysis of genotoxic effects, counting aberrations in anaphase and telophase, showed that all
samples were significantly different compared to the positive control (metilmetansulfonat), while only one
sample of water from the Sava river was significantly different compared to the negative control.

Keywords: genotoxicity, Allium test, pollution, water quality

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