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IZOLOVANJE DIARILHEPTANOIDA IZ BILJNE VRSTE

ALNUS GLUTINOSA

Mentor Istraţivaĉ
Mr MIROSLAV NOVAKOVIĆ MILICA PUĐA, uĉenica II 1 razreda
IHTM, Univerzitet u Beogradu Farmaceutsko fizioterapeutske škole,
Donska 27-29, Beograd
REZIME

U ovom radu je prikazano izolovanje i odreĎivanje diarilheptanoida oregonina i metilhirsutanonola iz kore
drveta biljne vrste Alnus glutinosa (jova). Poznato je da kora drveta jove sadrži diarilheptanoide, jedinjenja koja
pokazuju antibakterijsko, anti-inflamatorno, antivirusno dejstvo na virus gripa i antioksidativno dejstvo. Ova

jedinjenja su našla značajnu primenu i u hemoterapiji. Ekstrakcija kore drveta jove je vršena tri puta, pomoću sistema
rastvarača metanol/hloroform (50:50). Dobijeni ekstrakt je razdvajan gradijentnim programom na koloni silika gela
sistemom rastvarača metilen-hlorid/metanol. Tok razdvajanja je praćen tankoslojnom hromatografijom na
aluminijumskim pločicama obloženim silika gelom dimenzija 20×20 cm. Na osnovu dobijenih Rf vrendosti su
formirane zbirne frakcije od kojih su analizirane AG 14-30 i AG 140-152. Zbirna frakcija AG 14-30 je razdvajana

pomoću semipreparativnog HPLC-a, po programu koji je dao 8 frakcija i posle snimljenih NMR spektara utvrĎeno
je da je prva frakcija AG 14-30/1 čisto jedinjenje-metilhirsutanonol. Zbirna frakcija AG 140-152 je prečišćavana
preparativnom tankolsojnom hromatografijom na razvučenim pločama silika gela. Snimljeni su NMR spektri
prečišćene frakcije i njihovom analizom spektara identifikovan je diarilheptanoid oregonin.

Ključne reči: Alnus glutinosa, diarliheptanoidi, oregonin, metilhirutanonol, NMR, HPLC.

SUMMARY

This research was focused on isolating and determination of diarylheptanoids, oregonin
and metilhirsutanonol from the bark of Alnus glutinosa (alder) plant species. It is known that
alder bark contains diarylheptanoids that show antibacterial, anti-inflammatory,

antiviral effect on influenza virus, and antioxidant activity. These compounds have found
considerable application in chemotherapy. Extraction of the bark of alder was performed three times, using
a solvent system methanol/chloroform (50:50). The obtained extract was
separated by gradient program on the silica gel column in solvent system methylene chloride-
methanol. Flow separation was monitored by thin layer chromatography on aluminum plates coated with silica
gel dimensions of 20 ×20 cm. On the basis of Rf values are formed multifractions of which are analysed AG 14-30

and AG 140-152. Multifraction AG 14-30 was separated by semipreparative HPLC, according to the program which
gave 8 fractions and after recorded NMR spectra it was clear that first fraction, AG 14-30/1 was pure compound–
methylhirsutanonol. Multifraction AG 140-152 was separated by preparative thin layer chromatography on silica
gel plates. NMR spectra of rafined fraction were recorded and after their analysis diarylheptanoid oregonin was
identified.

Kee words: Alnus glutinosa, diarylheptanoids, oregonin, methylhirsutanonol, NMR, HPLC.

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