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Thyroid gland structure after treatment of middle-aged male rats with chaste tree
oil

Silvija Milosavljević
Regionalni centar za talente Beograd 2

Introduction

Chaste tree (Vitex agnus-castus L.; VAC) is native to
theMediterreaneanand it’s applied as alternative medicine
for reduction of PMS symptoms and premenopausal
problems. Likewise, VAC is used for prevention and
treatment of symptoms associated with andopouse,
osteoporosis, benign prostate hyperplasia and prostate
cancer. Significant number of physiological alternations in
function of thyroid gland are associated with ageing,
hypothyroidism is most common. Aim of this study was to
evaluate does VAC essential oil affects the structure and
function of the thyroid gland and to analyse potentional
changes in thyroid gland work in animal model of
andropause.

Material and methods

Middle-aged male Wistar rats (16 months) were treated for
three weeks with VAC essential oil (dose of 60 mg/kg body Figure 2. Immunohistochemical staining of thyroid
weight). After decapitation, thyroids were excised and peroxidase (TPO) and iodide transporter (NIS) in control
prepared for further histo-morphometric investigations and (C) and VAC treated middle-aged male rats (VAC).
immunohistochemical staining of enzyme
thyroidperoxidase (TPO) and iodide transporter (NIS).The
concetration of TSH in serum was determined by RIA kit, Conclusion
while total T4 and T3 were determined by ELISA asseys.

It was demonstrated that VAC essential oil do affects
Results structure and function of the thyroid. Administration of
VAC essential oil leads to microfollicular changes in the
structure of thiroid, accumulation of NIS protein and
Thyroids of VAC treated groups have characteristic increase levels of T4, T3 and TSH in serum. Detected
microfollicular structure. The volume of follicular changes indicate increased transport of iodine in the
epithelium and activation index were siginificantly follicular epithelium, hence increased biosynthesis of
increased (p<0.05), while the volume of the colloid was thyroid hormones. This study clearly shows that the
significantly decreased (p<0.05). The level of TSH, T4 and essential oil VAC has a positive impact on the activity of
T3 in serum were significantly increased (p<0.05). Change the thyroid gland, and can be used in the prevention and
in accumulation and localization of NIS was detected in treatment of symptoms associated with andropause.
follicular epithelium, while accumulation and localization
of TPO was unchanged.
Literature

Šošić-Jurjević B. i sar. (2010). Subcutaneusly administrated
Vitex agnus-castus Essential oil modulates thzroid structure
and increases serum TSH and total thyroid hormone
levelsin middle-aged male rats. The 14th International
Thyroid Congress, September 11-16., Paris, France, 2010.
Abstracts, p-0303.
Ignjatović Dj.i sar (2012). Bioactivity of the essential oil
from berries of Vitex agnus castus in middle aged male rats.
Digest Journal of Nanomaterials and Biostructure. 7:1727-
1734.

Pantelić J. i sar (2013). Vitex agnus-castus essential oil
Figure 1. Serum levels of TSH (A), T 4 (B) and T 3 (C) in affects thyroid C cells and bone metabolism in middle-aged
control (C) and VAC treated middle-aged male rats (VAC). male rats. Acta Veterinaria. 63:23-35.