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Effects of disinfectants Peral S® analysis of disinfectants' bactecidial effects, were
and Sekusept® Pulver on used in research. In suspensional method, MRS
strains were inoculated in disinfectants' solutions
6
methicillin - resistant (final number 9x10 per mL) and efficiency was
staphylococci isolated from done on 5, 10, 15, 20, 30 and 60 minutes. In BS
EN 1276:1997 the final number of bacteria was
animals and humans 6x10 per mL and efficiency was done on 2, 5,
7
10, 15, 20 and 30 minutes.
Results
Introduction
Results show that Peral S ®
The appearance of methicillin-resistant concentration 0.2% and Sekusept ® Pulver
staphylococci (MRS) presents one of the biggest concentration 2% in suspensional method kill all
problems in human and veterinary medicine, MRS strains. However, BS EN 1276:1997
because they show resistance to all beta-lactamic method shows that Peral S ® concentration 0.2%
antibiotics and most other antibiotics that have wasn't effective in 2 minutes, because 6 of 15
clinical appliance. Because of effective strains survived disinfectant effect and even two
antibiotics’ limited choice, MRS strains cause survived the effect for 5 minutes. In BS EN
fatal infections on humans and animals. Also, 1276:1997 method Peral S ® concentracion
because of dramatic deterioration of 0.5% killed all strains in 2 minutes, but Sekusept
epidemiological MRS situation, World Health ® Pulver concentration 2% wasn't effective in 2
Organization (WHO) and World Health minutes, because 7 of 15 strains survived, but
Organization for Animals (OIE) recommend this disinfectant was effective in exposition time
decrease and severe control of antibiotic use and, of 5 minutes.
at the same time, increase of disinfection
measures in hospitals and on farms. How the
prevalence of MRS resistance on different Conclusion
disinfectants is increasing, the question is how
disinfectants affect methicillin – resistant BS EN 1276:1997 was more precise in
staphylococci. the evaluation of disnifectants' efficiency. Peral S
® concentration 0.5% in 2 minutes kills all
The goal of this research is to determine present MRS strains. If lower Peral S ®
if MRS strains can be exterminated by two concentrations were used the disinfection time
commercial products that are widely used in would last at least 10 minutes. Sekusept ®
Serbia for hands’ and instruments’ disinfection. Pulver in reccomended concentration of 2% is

effective in minimum of 5 minutes.
Materials and methods

MRS strains from ear, eye, skin and
nose smears originated from dogs, cats and
humans were used in this research.
Staphylococci have been identified by API ID 32
test (bioMerieux). Control group were strains
MRSA ATCC 43300, ATCC 33591 and
S.aureus ATCC 25923. The evidence that
isolated staphylococci belongs to MRS was the
presence of mecA gene using PCR. Antibacterial
activity of Peral S® (Midra Eko DOO, Serbia)
(0.2% and 0.5% concentrations) and Sekusept®
Pulver (Ecolab, Germany) (2% concentration)
was tested. Peral S ® is peracetic acid, while
Sekusept ® Pulver has combined chemical
composition. Qualitative suspension method and
BS EN 1276:1997, method for quantitative